· 别名 : 梭菌肽酶 A
· 英文名称 : CollagenaseIII
· CAS : 9001-12-1
· 储存条件 : −20℃
· 纯度 : 210u/mg
· 外观(性状) : 冻干粉
· 单位 : 支
用于消化组织中连接部分使其成为单个细胞,用于哺乳动物组织细胞解离
胶原蛋白酶 来源于溶组织梭菌
Collagenase from Clostridium histolyticum
purified by chromatography, Type III, 2-10 FALGPA
units/mg solid, ≥250 CDU/mg solid (CDU =
collagen digestion units), lyophilized powder
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium
per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether)
- N, N, N′,N′-tetraacetic
acid, beta-mercaptoethanol, glutathione, thioglycolic acid and
8-hydroxyquinoline.
Crude collagenases are mixtures of enzymes (mostly
proteases) secreted by C. histolyticum. Effective release of cells from tissue
requires the action of both collagenase enzymes and the neutral protease.
Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The
culture filtrate is thought to contain at least 7 different proteases ranging
in molecular weight from 68-130 kDa.
包装
Product sold on the basis of collagen digestion
units (CDU).
Preparation Note
Collagenase may be dissolved in TESCA buffer (50
mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37 °C) at 0.05-0.1 mg/mL concentration.
Unit Definition
One collagen digestion unit (CDU) liberates
peptides from collagen from bovine achilles tendon equivalent in ninhydrin
color to 1.0 μmole of leucine in 5 hours at
pH 7.4 at 37 °C in the presence of calcium ions. One
FALGPA hydrolysis unit hydrolyzes 1.0 μmole of
furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One
Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C.
One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per
min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be
used for the isolation of cells from many types of animal tissue. It may be
used for the disaggregation of human tumor, mouse kidney, human adult/fetal
brain, lung and many other tissues. It may also be used in liver and kidney
perfusion studies, for the digestion of pancreas, isolation of nonparenchymal
rat liver cells and for hepatocyte preparation.
type Type III
form lyophilized powder
purified by chromatography
solubility TESCA buffer (50 mM TES, 0.36 mM
Calcium chloride, pH 7.4): soluble0.05-0.1 mg/mL at 37 °C
foreign activity Clostripain, may contain trace
amount neutral protease <1 unit/mg solid
storage temp. −20°C
浓度 | |
规格 | 1g |
保存 | −20℃ |
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